Do there is a protocol or instructed antibodies to conduct ICC staining on my thoroughly differentiated ALI cultures?
Appraise preclinical test compounds and biologics for their talents to modulate the immune system.
Make contact with us to debate custom made assay options for a range of other cell kinds to suit your unique demands.
It is necessary to quench the dissociation reagent working with FACS buffer or stop medium, by adding at the very least precisely the same or double the quantity in the dissociation reagent.
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The readings can even be carried out before the society is evaluated for endpoint characterizations, like electrophysiology or airway marker expression.
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We haven't uncovered any efficiency dissimilarities in between coated and non-coated problems; having said that, collagen coating might make improvements to differentiation in certain donors, or if working with freshly isolated cells.
Generally, HSAECs cultured in PneumaCult™-ALI-S Medium will sort a completely differentiated cuboidal epithelium after 4 to five months of culture. Some donor variability may very well be predicted.
A minimal reaction volume of 50 µL is required for constant efficiency Together with the CellPore™ Transfection System.
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